Journal: Annals of Translational Medicine
Article Title: Exercise training combined with alprostadil improves myocardial infarction and coronary microcirculation disorder in aged rats by inhibiting mitogen-activated protein kinase (MAPK) signaling pathway activation
doi: 10.21037/atm-22-5763
Figure Lengend Snippet: Exercise training combined with alprostadil treatment promotes microangiogenesis in the myocardial tissue of MI rats. (A) The expression of CD34 of the rats in each group is detected by immunohistochemistry. (B) Western blot detects the expression of microangiogenesis-related proteins (VEGF, FGF, and TSP-1) in the cardiomyocytes of the rats in each group. N=5 per group. **, P<0.01, vs. Control; ## , P<0.01, vs. MI; && , P<0.01, vs. Trained/ALPR. MI, myocardial infarction; ALPR, alprostadil; VEGF, vascular endothelial growth factor; FGF, fibroblast growth factor; TSP-1, thrombospondin-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Article Snippet: The membranes were blocked with 5% non-fat dry milk for 1 h. Primary antibodies, including vascular endothelial growth factor (VEGF) (ab32152, Abcam, UK), fibroblast growth factor (FGF) (9740S, CST, USA), thrombospondin-1 (TSP-1) (37879, CST), phosphorylated-P38 (p-P38) (4970S, CST), extracellular signal-regulated kinase 1/2 (ERK1/2) (4695S, CST), p-ERK1/2 (4370S, CST), c-Jun-N-terminal kinase (JNK) (9252S, CST), p-JNK (9255S, CST), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (5174S, CST) were added for incubation overnight at 4 °C on a shaker.
Techniques: Expressing, Immunohistochemistry, Western Blot, Control